WebCyto-ID Green autophagy assay revealed that PP2 failed to induce autophagy in ATG5 KO cells. PP2 treatment decreased the percentage of cells in the S and G 2 /M phases among WT cells but had no effect on cell cycle distribution of ATG5 KO cells, which showed a high percentage of cells in the S and G 2 /M phases. WebCyto-ID Green Dye Staining Protocol. Cyto-ID Green dye (8 µl) is mixed with 4 ml 1X buffer; Cell sample is centrifuged and resuspended into 200 µl of prepared Cyto-ID Green dye; Cell sample is incubated for 30 min at …
Cyto Id Autophagy Detection Kit 2 0 Enzo Biochem Bioz
WebDec 22, 2024 · Immunofluorescent images of HCT116 and HT29 cells treated with the pre-Cur protocol. Hoechst 33,342 staining (blue) indicates nucleus and Cyto-ID Green staining (green) autophagy status. b. Flow cytometry analysis of autophagy in HCT116 cells pretreated with varied concentrations of curcumin for 24 h and then 20 μM of 5-Fu for 24 h. WebOct 18, 2024 · The green fluorescent Cyto-ID intensity was analyzed using a FACSCalibur (BD Biosciences, Bedford, MA, USA) at 488 nm. Lysosomes Detection with LysoTracker Red Staining. MLO-Y4 cells (1 × 10 4 cells/mL) were treated with NaF (0, 4, 8 mmol/L) for 24 h. The treated cells were incubated with LysoTracker Red, a lysosomotropic probe (50 … shutterstock log in to my account
Autophagy Detection Nexcelom Bioscience
WebThis kit uses a green fluorescent probe that reacts with autophagic vacuoles and is detectable by flow cytometry: Downstream tips ; Incase of Low CYTO-ID Green dye … WebJournal: Cell reports Article Title: β2-adrenergic receptor signaling regulates metabolic pathways critical to myeloid-derived suppressor cell function within the TME doi: 10.1016/j.celrep.2024.109883 Figure Lengend Snippet: β2-AR signaling in MDSCs increases autophagy (A) Histogram presentation of Cyto-ID staining of autophagy … WebIn this study, we developed a novel method using the Cellometer image-based cytometer in combination with Cyto-ID (®) Green dye for autophagy detection in live cells. The method is compared with flow cytometry by measuring macroautophagy in nutrient-starved Jurkat cells. Results demonstrate similar trends of autophagic response, but different ... shutterstock music license