Fl83b cells

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August undefined, 2024

WebFL83B cells were obtained from the Taiwan Bioresource Collection and Research Center (BCRC number: 60325) and cultured in F12K medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Gibco) at 37°C in a humidified atmosphere with 5% CO 2. To make the stock medium containing 4 mM OLA, sodium oleate was conjugated … WebJan 1, 2024 · FL83B cells were grown in F12K medium supplemented with 10% foetal bovine serum (FBS)(Biological Industries, Haemek, Israel) and 100 mg/L penicillin and streptomycin at 37 °C in a humidified atmosphere of 5% CO2. Cells induced lipid accumulation in hepatocytes using 0.5 mM oleic acid (OA) for 48 h, then 3–100 μM …

Pepsin-digested chicken-liver hydrolysate attenuates …

WebJul 13, 2024 · Characteristics. tissue/cell type: Liver. condition: Healthy. treatment: none. Treatment protocol. FL83B cells were incubated in 400 µM palmitate diluted in growth medium for 24 h. One percent BSA diluted in growth medium was used as control. Moreover, gene knockout was achieved by the CRISPR-Cas9 system, using non … WebJan 11, 2024 · d FL83B cells were cultured with different concentrations (as indicated) of PC (34:2) for 16 h, and the resulting effects on AhR expression were determined by western blots. Ratio to β-actin ... hilden pharmacy lisburn

Fucoxanthin attenuates fatty acid-induced lipid accumulation in FL83B …

WebDec 1, 2024 · Among the subfractions, EAF5-5 markedly enhanced glucose uptake in TNF- α -treated FL83B cells. The possible bioactive compounds of the EAF5-5 fraction that were identified by the HPLC assay include syringic acid, p -coumaric acid, and cirsimaritin. The bioactive compound with the best effect of increasing glucose uptake was p -coumaric … WebNormal mouse hepatocyte cell line FL83B (ATCC CRL-2390) was cultured in Ham’s F-12 K medium containing 10% fetal bovine serum (FBS), 100 mg/mL penicillin and 0.25 mg/mL … Web(F) Lipid content analysis of FL83B cells treated with 400 mM OA and different CLH concentrations by using Nile red stain. The data are given as mean ± SEM (n ¼ 6). hilden park accountants hildenborough kent

Inhibitory effects of dioscorea polysaccharide on TNF-α

Fucoxanthin attenuates fatty acid-induced lipid accumulation in FL83B …

WebJul 13, 2007 · FL83B cells, derived from mouse liver, were treated with insulin and membrane translocation of GRK2 was determined using immunofluoresecence and … WebMay 25, 2011 · Finally, the possible mechanism of DPS promoting insulin sensitivity in TNF-α-induced FL83B cells was investigated in this study. DPS may attenuate c-Jun N … smallworld geldtransfer cubaWebThe FL83B cells were treated with insulin for 5 or 10 min. GRK2 was labeled with anti-GRK2 antibody and Cy3-conjugated secondary antibody and the cells were observed under the microscope. Insulin treatment increased the translocation of GRK2 from cytosol to the plasma membrane ( Fig. 1A ). hilden pc reparatur

"WebMay 21, 2024 · Abstract. FAM83B has been recently identified as an oncogene, but its role in thyroid cancers (TC) is still unclear. We examined the expression of FAM83B and its … " - Fl83b cells

Fl83b cells

Characterization of the mouse liver cell line FL83B

WebMay 1, 2024 · FL83B cells were seeded on 6-well plates and incubated with 0.5 mM oleic acid for 48 h. The cells were treated with or without tomatidine (0–10 μM) for 24 h. The plate was washed and the cells fixed with formalin solution before staining with Oil Red O solution to assay oil droplets as described previously [25]. Using an inverted microscope ... WebIn another experiment, FL83B cells were challenged with oleic acid to induce lipid accumulation and treated with various concentrations of fisetin. Results: NAFLD mice treated with fisetin had decreased body weight and epididymal adipose tissue weight compared to NAFLD mice. Fisetin treatment also reduced liver lipid droplet and …

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WebJan 12, 2024 · FL83B cells were co-transfected with pDR1 and pRL-TK (Promega, Seattle, WA, USA) containing CMV promoter-controlled Renilla luciferase gene using fuGENE HD (Promega, Seattle, WA, USA), and incubated with serum free media for 24 hr. Then the cells were treated with FFA and AM as described above, and, another 24 hr later, the … WebFAM83A-H are newly identified oncogenes characterised by a conserved DUF1669 domain. FAM83B can substitute for Ras to promote malignant transformation. Ablation of …

WebJun 15, 2015 · Abstract We studied the effect of silymarin and dimercaptosuccinic acid (DMSA), a chelating agent that was administered individually or in combination against lead (Pb) toxicity in rats. Wistar rats (200 ± 20) were randomly divided into five groups. Group A served as a control. Groups B–E were exposed to 2000 ppm of lead acetate in drinking … Web2.3. FL83B cell culture First, the culture method of FL83B cells was slightly modiﬁed from the American Type Culture Collection (ATCC) [18]. The short- and long-term impacts of CLHs on the survivability of FL83B cells in fetal bovine serum (FBS) containing culture sys-tem were determined (Fig. 1A), which could monitor

WebApr 25, 2024 · Briefly, FL83B cells (~ 18 × 10 6) were collected by centrifugation and sonicated in cold diluted assay buffer. The supernatant was removed, and cells were re-suspended in cold diluted assay buffer. BHB standard wells and sample wells were prepared as per the protocol. The reaction was initiated by adding developer solution. WebApr 1, 1973 · FL83B is a normal cell line of hepatocytes from a C57BL/6J mouse of 15–17 days of gestation (Breslow et al., 1973). FL83B required F-12 K medium with 10% FBS. …

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WebAug 4, 2024 · Cytotoxicity of PG/Dox synergism against normal cells. To access putative toxicity from PG/Dox synergism in liver, kidney, and heart, FL83B (murine normal hepatocyte), HK-2 (human kidney proximal tubule epithelial cell), and h9c2 (murine normal cardio-myoblast) were sequentially treated with PG and Dox for 12 h, respectively. hilden pharmacy smallworld game storageWebAug 1, 2007 · The present study was designed to test the hypothesis that GRK2 negatively regulates IR signaling. FL83B cells, derived from mouse liver, were treated with insulin and membrane translocation of ... hilden park golf centreWebA mouse HCC cell line (Hepa1-6) and a mouse hepatocyte line (FL83B) were used to elucidate the mechanism Cite 6th Dec, 2024 Mohamed Elkablawy Taibah University and … smallworld game tutorialWebFL83B is a hepatocyte cell line derived in 1969 by Charity Waymouth at The Jackson Laboratory, Bar Harbor, Maine from a normal liver taken from a 15-17 day old fetal … hilden park golf courseWebElk-3 siRNA at 20 nM was transfected into TGF-β1-treated FL83B cells (A) or AML12 cells (B). At 48 hours posttransfection, the cells were harvested and lysed. Western blot … smallworld documentationWebDec 29, 2015 · FL83B cell line represent normal hepatocytes whereas hHSC cell line has the angiotensin 1 receptors which is the main target for losartan receptors. Confocal … hilden psychotherapie