WebMethods: HCC cell lines HepG2 and HuH-7 were tested. Cell viability was examined using MTT assay and cell invasion was determined with Transwell migration assay. The … WebSN-38 (NK012) is an active metabolite of CPT-11, inhibits DNA topoisomerase I, DNA synthesis and causes frequent DNA single-strand breaks. SN-38 induces autophagy. SN …

Research Paper Synergistic Antitumor Effect of Sorafenib in …

Web10 apr. 2024 · The effects of AT-1976 on chemoagent-induced cell toxicity were examined. For XAF1-positive HepG2 cells, AT-1976 co-treatment reduced the IC50 for CPT-induced cell toxicity from 0.12 μM to 0.026 μM (Figure 6A), while there was no significant difference in IC50 for XAF1-negative Hep3B cells with or without the presence of AT-1976 (Figure 6B). http://html.rhhz.net/ZGYLXTB/html/201612024.htm nissan motor corp

Kaempferide exhibits an anticancer effect against hepatocellular ...

Human hepatocytes were prepared from liver biopsies obtained from two patients (one female and one man, aged 43 ± 6.0 years) submitted to surgical resection for liver tumors after obtaining patients’ written consent. The study protocol was approved by the Ethical Committee of the Institution. The … Meer weergeven Sorafenib (FS10808), Regorafenib (FR16116), Cabozantinib (FD59688), and Lenvatinib (FC75063) were obtained from Carbosynth … Meer weergeven Cell proliferation was assessed in 3D and 2D cultured cells. Non-trypan blue-stained viable cells were quantified in trypsin-treated spheroids, as well as Ki67-positive cells were determined in 4% paraformaldehyde … Meer weergeven Mitochondrial respiration in intact adhered cells was assessed with the XFp Cell Mito Stress Test Kit in a XFp Extracellular Flux Analyzer (Seahorse Bioscience, Billerica, Massachusetts, USA). Oxygen consumption … Meer weergeven Cell death was assessed either in trypsin-isolated cells with the measurement of trypan blue-stained non-viable cells in spheroids, or … Meer weergeven Web27 dec. 2024 · EC 50 values (µg/mL) were calculated and indicate the effective concentration at which the absorbance was 0.5 for reducing power. Cell lines and culture … Web2 okt. 2024 · The IC50 of resistant cell lines was determined by an MTT assay, and the resistance index (RI) was further calculated as the IC50(resistant cells)/IC50(parental cells). nuova health knee support