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P53 activation by knockdown technologies

WebApr 1, 2024 · In response to cellular stresses, p53 transcriptionally activates or represses a number of downstream target genes, which, in turn, participate in the modulation of multiple biological processes including cell cycle arrest, programmed cell death, cellular senescence, DNA repair, oxidative response, and metabolic regulation ( 6 ). WebSENP1 interacts with p53 and deSUMOylates p53 in cells and in vitro. Knockdown of SENP1 markedly induced p53 transactivation activity. We further show that SENP1 depletion synergizes with DNA damage-inducing agent etoposide to induce p53 activation and the expression of p21, leading to synergistic growth inhibition of cancer cells.

An unexpected role for p53 in regulating cancer cell ... - Science

WebJul 11, 2014 · Here, we found that HRP-3 knockdown regulates the radioresistance of p53-null H1299 cells through a distinctly different molecular mechanism. HRP-3 depletion was sufficient to cause apoptosis of H1299 cells by generating substantial levels of reactive oxygen species (ROS) through inhibition of the Nrf2/HO-1 antioxidant pathway. WebFeb 20, 2012 · Knockdown of Prpf8 or UBL5 caused accumulation of p53, elevated p53-responsive transcriptional reporter activity and an increase in the mRNA and protein levels … brook hollow apartments new haven https://mallorcagarage.com

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WebJan 1, 2016 · Results. Knockdown of the UGT1A proteins by RNAi in human colon cancer cells and knockout of the Ugt1 locus in intestinal crypt stem cells reduces phosphorylated p53 activation and compromises the ability of p53 to control apoptosis. Targeted deletion of intestinal Ugt1 expression in Ugt1 ΔIEC mice represses colon inflammation-induced p53 … WebOct 5, 2024 · Fig. 1: c-Myc activates the pan-cancer lncRNA MILIP to repress p53. a c-Myc bound to MILIP promoter in A549 and MCF-7 cells. An E-box motif, not associated with MYC target genes on Chr22, was... WebMay 25, 2007 · Introduction Morpholino phosphorodiamidate oligonucleotides (MOs) [1] and short inhibitory RNAs (siRNAs) [2] have been instrumental to induce sequence-specific … care coordination certification online

Zebrafish Models of p53 Functions - CSHL P

Category:p53 Activation by Knockdown Technologies – ScienceOpen

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P53 activation by knockdown technologies

p53 Activation by Knockdown Technologies – ScienceOpen

WebMar 23, 2024 · p53 signaling is activated by PCA3 knockdown To assess whether PCA3 -mediated chromatin rearrangement altered gene expression, we performed gene expression profiling of C4-2 cells transfected... Webdependent cell death pathway. p53 activation has also recently been shown to be an unspecified off-target effect of siRNAs. Both commonly used knockdown technologies …

P53 activation by knockdown technologies

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WebFinally, nonspecific activation of p53 has been described in siRNA-treated cells (Scacheri et al. 2004), suggesting that inhibition of p53 may be effective in reducing the off-target effects of other knockdown technologies (Robu et al. 2007).

WebApr 17, 2013 · Restoration of p53 functionality and activation of p53 to improve the efficacy of tumor therapy have attracted much attention within many different tumor entities and have demonstrated superior cell death induction for many combinations [3, 13, 16].In contrast, the presence or activation of p53 within certain therapeutic settings was shown … WebJan 28, 2013 · Our study showed that knockdown of these p53 mutants by small interfering (si)RNA not only induced cell cycle arrest and cell apoptosis in T24 and 5637 bladder cancer cell lines, but also cooperated with cisplatin in the inhibition of these cancer cells, suggesting that siRNAs may serve as alternative agents for the treatment of bladder …

WebMar 24, 2012 · HIF-2α knockdown, but not HIF-1α knockdown, increases the activation of p53. Finally, inhibition of HIF-2α blocks arsenite-induced proliferation and malignant transformation. WebApr 10, 2007 · Search worldwide, life-sciences literature Search. Advanced Search

WebBoth commonly used knockdown technologies can thus induce secondary but sequence-specific p53 activation. p53 inhibition could potentially be applicable to other systems to …

WebJan 3, 2013 · p53 is activated in response to disruption of the pre-mRNA splicing machinery. In this study, we show that interfering with the splicing machinery results in activation of … care coordination for the homelessWebJan 8, 2009 · We report here that a deficiency in L11 activates the p53 pathway, and absence of L11 disrupts the normal embryonic development of zebrafish, presumably through a p53-mediated apoptotic response. Results Knockdown of rpl11 leads to morphological defects mainly in the developing brain care consultancy choiceWebMay 25, 2007 · Morpholino phosphorodiamidate antisense oligonucleotides (MOs) and short interfering RNAs (siRNAs) are commonly used platforms to study gene function by … brookhollow apartments desoto tx