Phleomycin plates
WebbDescription Zeocin™ Selection Reagent is a formulation of phleomycin D1, a basic, water-soluble, copper-chelated glycopeptide isolated from Streptomyces verticillus. The … Webb1 feb. 2002 · Selection on phleomycin plates gave rise to resistant fungal colonies only when transformed A. tumefaciens was used for cocultivation together with AS, indicating that induction of the vir-genes was essential for T-DNA transfer between A. tumefaciens and hyphae of ecto- mycorrhizal fungi.
Phleomycin plates
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Webb5-alpha Competent E. coli cells were transformed with 2 µl of the reaction sample following the Gibson Assembly Transformation Protocol from NEB. Next, 100 µl of the cells were plated onto LB medium plates with Phleomycin at a concentration of 20 ug/ml. The plates were incubated overnight at 37 °C. Figure 9. Webbnote: phleomycin is also available from Sigma, and zeomycin (trade name ZeocinTM) is also available from Invitrogen ... • Gently resuspend the cells in 0.5ml TAP medium and …
Webbplates (chemically transformed cells). Test the medium adjusted to pH values ranging from 6.5–8.0 and select the pH that allows you to use lowest Zeocin™ concentration. … Webb1 jan. 2012 · GYE agar plates containing 3 μg/mL of phleomycin (GYE-Phl): Prepare and autoclave 500 mL GYE agar as described in Subheading 2, item 1. Add 0.5 mL of 3 mg/mL phleomycin stock solution into the warm GYE agar and dispense 10 or 20 mL into each 60- × 15-mm or 100- × 15-mm Petri plate, respectively.
WebbThe phleomycin-resistant cells ( Fig. 9.6) were obtained in an average of 1/1.6 plates through selection for 3–4 weeks, with a transformation efficiency of approximately 5.5 × … Webbfrom the original plate selected on 50 µg/ml of phleomy-cin maintained resistance to phleomycin. Conversely, 11 of 18 colonies selected on 100 µg/ml of phleomycin …
Webbhygromycin B and phleomycin were purchased from Cayla. For efficient selection, some batches of hygromycin B required higher concentrations (up to 400 µg/ml). Phleomycin …
Webb15 juli 2014 · Phleomycin化学特性 CAS号：11006-33-0 分子式：C55H85O21N20S2Cu-HCl 分子量：1525 推荐使用浓度 大肠杆菌 Phleomycin抗性的转化子可以在低盐LB琼脂糖培养基 (yeast extract 5g/l, Tryptone 10g/l, NaCl 5g/l, Agar 15 g/l, pH 7.5)，5ug/ml Phleomycin的条件下筛选。 酵母 10 μg/ml Phleomycin 真菌 根据转化子对Phleomycin 的敏感性不同， … date a live season 4 sub indoWebb29 apr. 2024 · Given phleomycin causes global DSBs by an unknown mechanism, we also tested the effects of deletion of DMA1 and DMA2 on plates containing an established … bitwarden import passwords edgeWebbSerial dilutions of each strain were plated onto YEPD, MMS plates (0.003% and 0.004%), and phleomycin plates (3 g/ml and 6 g/ml). To measure UV sensitivity, ... bitwarden import icloud keychainWebb15 mars 2002 · Selection for phleomycin resistance was performed on YPD plates containing 7.5 µg/ml phleomycin (PHLEL0100; Cayla, France) (dissolve in water and add … date a live season 5 release date 2022Webb21 jan. 2003 · The population of strains from phleomycin plates displayed a significant increase in mean lovastatin yield (P < 0.001 in a bootstrap confidence interval for … bitwarden import from excelWebb1 jan. 2002 · 1. (A) Two-step gene disruption. An altered form of the target gene (e.g., carrying a single base mutation) present on a circular plasmid with a selectable gene (e.g., URA3) is integrated into the yeast genome by homologous recombination, selecting for inheritance of the selectable gene (e.g., Ura+). This generates a duplication of the target ... bitwarden import passwords from chromeWebbPhleomycin ready made solution 0.2 mum filtered, 20mg/mL in 20mM HEPES MilliporeSigma …is a glycopeptide antibiotic produced by Streptomyces verticillus. It is a member of Bleomycin family with wide spectrum antibiotic activity in both prokaryotic and eukaryotic cells. date a live spirit pledge download