WebSep 9, 2010 · Various forms of stress induce pathways that converge on the phosphorylation of the alpha (α) subunit of eukaryotic translation initiation factor eIF2 at serine 51 (S51), a modification that results in a global inhibition of protein synthesis. ... WebTHUNDER™ Phospho-eIF2α (S51) + Total eIF2α TR-FRET Cell Signaling Assay Kit. Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-EIF2α (S51) and Total eIF2α in cell lysates. This unique kit allows to simultaneously detect phosphorylated and total eIF2α proteins from the same lysate sample in separate wells within the ...
Phospho-EIF2S1 (Ser52) Polyclonal Antibody - Thermo …
WebPharmaco effects of GSK2606414 on Total and Phospho (Ser52) EIF2a. HepG2 and C2C12 cells were cultured and treated with increasing concentrations of GSK2606414, a selective PERK inhibitor, before activation with 500 nM Thapsigargin. After medium removal, the cells were lysed and 16 µL of lysate were transferred twice over into a low volume ... WebHuman Phospho-eIF2 alpha (S52) Antibody Summary. Detects human Phospho-eIF2 alpha (S52) in ELISAs and Western blots. Lyophilized from a 0.2 μm filtered solution in PBS with … fly haikyuu song
Phospho-EIF2S1 (Ser51) Recombinant Polyclonal Antibody …
WebNov 2, 2024 · Eukaryotic initiation factor eIF2 is a key component of the ternary complex whose role is to deliver initiator tRNA into the ribosome. A variety of stimuli, both physiologic and pathophysiologic activate eIF2 kinases that phosphorylate the α subunit of eIF2, preventing it from forming the ternary complex, thus attenuating cellular protein … WebPhosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. eIF2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by … WebThe PathScan ® Phospho-eIF2α (Ser51) Sandwich ELISA Kit #7286 detects endogenous levels of eIF2α protein when phosphorylated at Ser51 as shown in Figure 1. Kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. fly hadley