Phospholipidosis detection reagent

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August undefined, 2024

WebThe HCS LipidTOX Red phospholipidosis detection reagent was developed to characterize the potentially toxic side effects of compounds on lipid metabolism in mammalian cell … WebInvitrogen™ HCS LipidTOX™ Green Phospholipidosis Detection Reagent (1000X), for cellular imaging. Easily detected by fluorescence microscopy. Manufacturer: Invitrogen™ …

Drug-Induced Phospholipidosis: Best Practices and …

WebSeveral methods for the detection of phospholipids have been reported (29-37). They are summarized in Table 1. In-vitro screening assays using the fluorescently tagged lipid NBD-PE, the fluorescent probe Nile red as well as the phosphatidylcholine-conjugated dye NBD-PC were previously used to determine intracellular phospholipids (29, 30, 34). WebInvitrogen’s HCS LipidTOX™ Phospholipidosis and Steatosis Detection Kits offer the reagents for performing image-based high-content screening (HCS) assays to … the prize winner of defiance ohio film

Predicting in vivo phospholipidosis-inducing potential of drugs by …

WebThermo Fisherhcs lipidtox green phospholipidosis detection reagent Hcs Lipidtox Green Phospholipidosis Detection Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more Webreagents containing DMSO using equipment and practices appropriate for the hazards posed by such materials. Dispose of the reagents in compliance with all pertaining local … WebMar 17, 2024 · This chapter describes a cell-based high-content phospholipidosis assay using the LipidTOX reagent in a high-throughput screening (HTS) platform. This assay … signalarity research labs

Cell-Based Imaging Assay for Detection of Phospholipidosis

Drug-induced phospholipidosis caused by combinations of ... - 豆 …

WebJan 27, 2024 · Briefly, cells were incubated in complete bronchial epithelial growth medium supplemented with 1 x phospholipidosis detection reagent for 48 hr. Cells were subsequently fixed in 4% PFA and stained with 1 X neutral lipid detected reagent for 30 min and counterstained with 1 μg mL-1 Hoechst 33258. Staining intensity was normalized to … WebCell Based Assays Bioassays, kits, and reagents used to analyze various properties of living cell samples. Products include apoptosis assays, signal transduction assays, and cell growth and differentiation assays. General Cell Based Assays (1,186) Signal Transduction Reagents and Kits (1,016) Cell Growth and Differentiation Reagents and Kits (423) signal app windows s modeWebSep 3, 2012 · Drug-induced phospholipidosis (PLD) is a lysosomal storage disorder characterized by the accumulation of phospholipids within the lysosome. ... After 48 h, the medium was replaced with fresh medium that included the test substances and HCS LipidTOX Green phospholipidosis detection reagent (Invitrogen, San Diego, US) at their … the prize winner of defiance ohio cast

"WebHCS LipidTOX™ Phospholipidosis and Steatosis Detection Kit offers a complete set of reagents for performing image-based high-content screening (HCS) assays to characterize phospolipidosis and steatosis, toxic side effects on lipid metabolism that can be triggered by drugs or other compounds. " - Phospholipidosis detection reagent

Phospholipidosis detection reagent

Palmitate induces cisternal ER expansion via the activation of XBP …

WebThe EM method, the gold standard of detecting cellular phospholipidosis, has downsides, namely, low-throughput, high-costs, and unsuitability for screening a large chemical library. This chapter describes a cell-based high-content phospholipidosis assay using the LipidTOX reagent in a high-throughput screening (HTS) platform. WebPhospholipidosis is an excessive intracellular accumulation of phospholipids and drug, which is normally reversible after discontinuation of drug treatment. Currently, it is …

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WebAmiodarone is a widely used antiarrhythmic drug known to induce phospholipidosis. This simple enzymatic screen can be performed without sophisticated instruments and is scalable for high throughput drug screening. The full study of identifying drugs’ phospholipidosis property using the LPLA2 Inhibitor Screen can be found here. WebOct 30, 2024 · In this chapter, we describe fluorescent reagents and the protocols for the measurement of various parameters such as mitochondrial membrane potential, plasma …

WebHCS LipidTOX™ Phospholipidosis Detection Reagents 3 Preparing the Labeling Solution and Staining the Cells 1.1 Dilute the 1000X LipidTOX™ phospholipidosis detection … WebJun 6, 2024 · The phospholipid storage was accompanied by an expansion of the endolysosomal compartment in cultured TEC as evidenced by the increased intensity of LysoTracker Red staining and the enhanced...

WebThe HCS LipidTOX™ Red phospholipidosis detection reagent was developed to characterize the potentially toxic side effects of compounds on lipid metabolism in mammalian cell … WebNov 9, 2024 · Phospholipidosis (PLD), the intracellular accumulation of phospholipids, is an adaptive response to toxic stimuli and serves as an important parameter in the biological …

Webdrug-induced phospholipidosis. The morphological hallmark of phospholipidosis is the appearance of unicentric or multicentric-lamellar bodies when viewed under an electron …

WebMar 1, 2024 · LipidTOX Green Phospholipidosis Detection Reagent (LipidTOX, Invitrogen, Carlsbad, CA, USA) was applied to confirm induction of phospholipidosis by selected drugs. According to the manufacturer's protocol, LipidTOX was diluted in full media (1:500) and then filtered with a syringe filter (2 μm in pores size). ... theprizmagamingWebThe LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be detected by fluorescence microscopy or an HCS reader. This probe is compatible with the HCS LipidTOX™ phospholipidosis detection reagents (H34350, H34351). HCS LipidTOX™ neutral lipid stains can also be used to monitor the formation and ... the priz folkestoneWebInitial experiments focused on the response of human mesothelioma cells to agents known to cause drug-induced phospholipidosis. When H-meso cells were treated with various concentrations of either chloroquine or verapamil a dose dependent increase in Lyso-ID Red signal is observed (Figure 5). signal app windows storeWebDec 7, 2024 · Annexin V-FITC/PI apoptosis detection kit was acquired from BD (Franklin Lakes, NJ, USA). Then, Hoechst 33342, eBioscience™ JC-1 Mitochondrial Membrane Potential Dye, Cell ROX Deep Red, and HCS LipidTOX™ Green Phospholipidosis Detection Reagent were obtained from Thermo Fisher Scientific (Waltham, MA, USA). the prize winner of defianceWebThe EM method, the gold standard of detecting cellular phospholipidosis, has downsides, namely, low-throughput, high-costs, and unsuitability for screening a large chemical library. This chapter describes a cell-based high-content phospholipidosis assay using the LipidTOX reagent in a high-throughput screening (HTS) platform. the prize winner of defiance ohWebThis probe is compatible with the HCS LipidTOX phospholipidosis detection reagents. HCS LipidTOX neutral lipid stains can also be used to monitor the formation and differentiation of adipocytes, a process called adipogenesis. Cell Analysis, Cellular Imaging, High-Content Screening (HCS) Order Info Shipping Condition: Room temperature Specifications signal arrived during cgo executionWebLipidTox Green neutral lipid stain and LipidTox Red phospholipidosis detection reagent (Kit from Molecular Probes) ... ORIGINAL ARTICLE 232 E-LDL and Ox-LDL Differ in the Lipid Storage. phospholipidosis detection depending on the emission spectra of the dye (Table 1) (30,37–39). Lipid-loaded macrophage foam cells play a major role in the prize-winning poem wrote by rizal