site stats

Psin-ef2 vector

WebLa fusariose vasculaire du cyclamen est une maladie causée par le champignon tellurique Fusarium oxysporum f. sp. cyclaminis. Elle est considérée comme l’une des maladies les plus graves du cyclamen et se traduit par des pertes atteignant jusqu’à 50 % … WebMay 11, 2024 · abstract type AbstractPolynomial end """ Polynomial <: AbstractPolynomial Polynomials written in the canonical form --- Polynomial(v::T) where T<:Union{Vector{<:Real}, NTuple{<:Any, <:Real}}) Construct a `Polynomial` from the list of the coefficients. The coefficients are assumed to go from power 0 in the ascending order.

Solución numérica de problemas elípticos y parabólicos por …

WebFull Sequences from Depositor (1) Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results. Discrepancies between … http://www.labbase.net/Supply/SupplyItems-4229347.html townhomes georgetown tx for rent https://mallorcagarage.com

Addgene: pSin Helper

WebApr 27, 2024 · The PCR and sequencing analyses confirmed that the DAB2IP gene sequence was consistent with the sequence in GenBank (NCBI, NM_138709). Stably transfected … WebApr 6, 2024 · Briefly, the pSin-EF2-TIPE3-HA or empty vector, as well as the lentivirus packaging plasmids psPAX2 and pMD2.G, were co-transfected into 293FT cells using the calcium phosphate method. After... townhomes georgetown sc

pSin-EF2-Oct4-Pur Addgene 16579 product information

Category:Fusarium wilt of cyclamen : early detection and biocontrol

Tags:Psin-ef2 vector

Psin-ef2 vector

Psin Ef2 Lin28 Pur addgene inc Bioz

WebpSin (Search Vector Database) Vector type Synthetic Biology Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) NEB Stable Copy number Low Copy Gene/Insert Gene/Insert name Sindbis Glycoproteins Cloning Information Cloning method Unknown Resource Information Supplemental Documents WebPsin Ef2 Puromycin Lentiviral Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article …

Psin-ef2 vector

Did you know?

WebOct 19, 2024 · The pSin-EF2-puro-ZNF671-HA or pSin-EF2-puro-vector plasmids were obtained from Land. Hua Gene Biosciences (Guangzhou, China); pSin-EF2-puro-Vector … WebJan 22, 2015 · The pri-miR-34c sequence was cloned into the lentiviral plasmid pSin-EF2-puromycin (Addgene, Cambridge, MA, USA); pSin-EF2-miR-34c or negative control empty …

WebThe psPAX2 packaging plasmid and pMD2.G envelope plasmid can be used with this vector. These plasmids were created by your colleagues. Please acknowledge the Principal … WebFeb 3, 2024 · Lentiviral vector construction, virus infection and the establishment of stable cell line. The AKR1B10 CDS (coding DNA sequence) was cloned into the lentiviral …

WebOct 5, 2024 · To determine that whether LASP1 is a direct target of miR-203a-3p, CNE-2 and SUNE-1 cells were co-transfected with miR-203a-3p mimic or miR-Ctrl (50 nM, RiboBio) and the pSin-EF2-puro-LASP1 (LASP1) or empty pSin-EF2-puro-Vector (Vector) (2 μg; Addgene, Cambridge, MA, USA). The cells were harvested for assays 48hs after transfection. WebJul 18, 2024 · The full length of CTBP1-DT was generated by subcloning the PCR-amplified human CTBP1-DT sequence and cloned into pSin-EF2-vector. The DDUP ORF with FLAG-tag expression plasmid was generated by cloning the full length of CTBP1-DT.

WebNov 1, 2024 · The pSin-EF2-empty vector or pSin-EF2-CES2 plasmids and the lentivirus packaging plasmids (pMD2.G and psPAX2) were co-transfected into 293FT cells using the calcium phosphate method, as previously described [14]. Lentivirus particles were harvested and infected into the CAL33 and UM1 cells. The vector or CES2 stably transfected cells …

WebThe pSin-EF2- GFP-Puro (EF1 a promoter) provided maximal GFP expression in both the undifferentiated cells as well as the day 10 differentiated cells with greater than 95% of … townhomes germantownWebConstructs expressing eGFP from the pSin- EF2-GFP-Puro based vector were used in subsequent studies. A key bottleneck in many stem cell applications is the ability to identify, select or ... townhomes gilbertWebSep 14, 2024 · The empty vector pSin-EF2-Pur was used as a control. The plasmids were verified by sequencing. For stable knockdown of ELMO1, GV248-puro [Shanghai Genechem Co., Shanghai, China] containing hairpin sequence targeting ELMO1 or a control plasmid [control hairpin] was co-transfected with psPAX2 and pMD2G [Addgene, Cambridge, MA, … townhomes germantown tn