Psin-ef2 vector
WebpSin (Search Vector Database) Vector type Synthetic Biology Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) NEB Stable Copy number Low Copy Gene/Insert Gene/Insert name Sindbis Glycoproteins Cloning Information Cloning method Unknown Resource Information Supplemental Documents WebPsin Ef2 Puromycin Lentiviral Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article …
Psin-ef2 vector
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WebOct 19, 2024 · The pSin-EF2-puro-ZNF671-HA or pSin-EF2-puro-vector plasmids were obtained from Land. Hua Gene Biosciences (Guangzhou, China); pSin-EF2-puro-Vector … WebJan 22, 2015 · The pri-miR-34c sequence was cloned into the lentiviral plasmid pSin-EF2-puromycin (Addgene, Cambridge, MA, USA); pSin-EF2-miR-34c or negative control empty …
WebThe psPAX2 packaging plasmid and pMD2.G envelope plasmid can be used with this vector. These plasmids were created by your colleagues. Please acknowledge the Principal … WebFeb 3, 2024 · Lentiviral vector construction, virus infection and the establishment of stable cell line. The AKR1B10 CDS (coding DNA sequence) was cloned into the lentiviral …
WebOct 5, 2024 · To determine that whether LASP1 is a direct target of miR-203a-3p, CNE-2 and SUNE-1 cells were co-transfected with miR-203a-3p mimic or miR-Ctrl (50 nM, RiboBio) and the pSin-EF2-puro-LASP1 (LASP1) or empty pSin-EF2-puro-Vector (Vector) (2 μg; Addgene, Cambridge, MA, USA). The cells were harvested for assays 48hs after transfection. WebJul 18, 2024 · The full length of CTBP1-DT was generated by subcloning the PCR-amplified human CTBP1-DT sequence and cloned into pSin-EF2-vector. The DDUP ORF with FLAG-tag expression plasmid was generated by cloning the full length of CTBP1-DT.
WebNov 1, 2024 · The pSin-EF2-empty vector or pSin-EF2-CES2 plasmids and the lentivirus packaging plasmids (pMD2.G and psPAX2) were co-transfected into 293FT cells using the calcium phosphate method, as previously described [14]. Lentivirus particles were harvested and infected into the CAL33 and UM1 cells. The vector or CES2 stably transfected cells …
WebThe pSin-EF2- GFP-Puro (EF1 a promoter) provided maximal GFP expression in both the undifferentiated cells as well as the day 10 differentiated cells with greater than 95% of … townhomes germantownWebConstructs expressing eGFP from the pSin- EF2-GFP-Puro based vector were used in subsequent studies. A key bottleneck in many stem cell applications is the ability to identify, select or ... townhomes gilbertWebSep 14, 2024 · The empty vector pSin-EF2-Pur was used as a control. The plasmids were verified by sequencing. For stable knockdown of ELMO1, GV248-puro [Shanghai Genechem Co., Shanghai, China] containing hairpin sequence targeting ELMO1 or a control plasmid [control hairpin] was co-transfected with psPAX2 and pMD2G [Addgene, Cambridge, MA, … townhomes germantown tn