WebbRiboGreen 试剂不会检测到游离核苷酸对样品存在的污染，因此可以更准确地测量降解样品中的完整 RNA 量。 RiboGreen 试剂尤其适于在生成 cDNA、进行 northern 印迹分析、S1 核酸酶检测、RNase 保护检测、逆转录 PCR 和差异显示 PCR 之前快速准确测量 RNA。 WebbRiboGreen ® Low Concentrations ... The report also contains a table with the corrected ODs, calculated concentrations and standard deviation and % CV of the replicate sets. Absorbance 14 Direct Oligonucleotide Quantitation Basis for the Assay: The aromatic ring structure of the purine and pyrimidine moieties that make up the
Impact of lipid nanoparticle size on mRNA vaccine immunogenicity
WebbThe riboso- mal RNA standard, provided at 100 µg/mL in the RiboGreen Kit, can simply be diluted 50-fold in TE to make the 2 µg/mL working solution. For example, 40 µL of the RNA standard mixed with 1.96 mL of TE will be sufficient for … Webb7 apr. 2024 · Ribogreen reagent was then added to each well and fluorescence was measured using GloMax ® Explorer (Promega, Madison, WI, USA). A standard curve was generated (0.1–2.5 ng/µL final RNA concentration) in the presence of Triton X-100. hind traducere
RNA Quantitation by Fluorescence-Based Solution Assay: …
Webb简介：厂家直销RNA荧光 (RiboGreen)定量检测试剂盒，RNA荧光 (RiboGreen)定量检测试剂盒采用的技术是经过精心研制、成功实验证明的权威而经典的技术方法，收到货后，应按照产品说明书上的规定妥善保管并在有效期内使用。 ... 报价：18317267598 QQ索取说明书 产品详细 RNA荧光 (RiboGreen)定量检测试剂盒产品说明书（通用版） 产品规格 20次，我 … WebbThe Quant-iT ™ RiboGreen RNA Reagent enables quantitation of as little as 1 ng/mL RNA (200 pg RNA in a 200 µL assay volume) with a fluorescence microplate reader using … WebbI am trying to perform a RiboGreen Assay to calculate mRNA encapsulation efficiency. Triton buffer (1× TE buffer pH 7.5 with 2 % Triton X-100) was prepared and tested, but … homemade hamburger and noodles